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Assessing the zearalenone-binding activity of adsorbent materials during passage through a dynamic in vitro gastrointestinal model

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Author: Avantaggiato, G. · Havenaar, R. · Visconti, A.
Institution: TNO Voeding
Source:Food and Chemical Toxicology, 10, 41, 1283-1290
Identifier: 237299
doi: doi:10.1016/S0278-6915(03)00113-3
Keywords: Health · Physiological Sciences · Activated carbon · Cholestyramine · Gastrointestinal model · Mycotoxins · Zearalenone · activated carbon · adsorbent · colestyramine · zearalenone · anion exchange resin · charcoal · article · binding affinity · digestion · food contamination · model · small intestine absorption · swine · wheat · absorption · animal · bile · biological model · chemistry · digestive system · intestine absorption · metabolism · pancreas · physiology · Animalia · Triticum aestivum · Absorption · Animals · Anion Exchange Resins · Bile · Charcoal · Cholestyramine · Digestive System · Food Contamination · Intestinal Absorption · Models, Biological · Pancreas · Swine · Triticum · Zearalenone


A novel approach is presented herein to study the intestinal absorption of mycotoxins by using a laboratory model that mimics the metabolic processes of the gastrointestinal (GI) tract of healthy pigs. This model was used to evaluate the small-intestinal absorption of zearalenone from contaminated wheat (4.1 mg/kg) and the effectiveness of activated carbon and cholestyramine at four inclusion levels (0.25, 0.5, 1 and 2%) in reducing toxin absorption. Approximately 32% of ZEA intake (247 μg) was released from the food matrix during 6 h of digestion and was rapidly absorbed at intestinal level. A significant reduction of intestinal absorption of ZEA was found after inclusion of activated carbon or cholestyramine, even at the lowest dose of adsorbents, with a more pronounced effect exhibited by activated carbon. In particular, when 2% of activated carbon or cholestyramine was added to the meal the ZEA intestinal absorption was lowered from 32% of ZEA intake to 5 and 16%, respectively. The sequestering effect of both adsorbents took place already during the first 2 h of digestion and persisted during the rest of the experiment. The GI-model is a rapid and physiologically relevant method to test the efficacy of adsorbent materials in binding mycotoxins and can be used to pre-screen mycotoxin/adsorbent combinations as an alternative to animal experiments. © 2003 Elsevier Ltd. All rights reserved.