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Metabolomics (liver and blood profiling) in a mouse model in response to fasting: A study of hepatic steatosis

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Author: Ginneken, V. van · Verhey, E. · Poelmann, R. · Ramakers, R. · Dijk, K.W. van · Ham, L. · Voshol, P. · Havekes, L. · Eck, M. van · Greef, J. van der
Type:article
Date:2007
Institution: TNO Kwaliteit van Leven
Source:Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 10, 1771, 1263-1270
Identifier: 240207
doi: doi:10.1016/j.bbalip.2007.07.007
Keywords: Analytical research · Biomedical research · Hepatic steatosis · Lipids · Liver · Metabolomics · Starvation · Triacylglycerols · cholesterol · cholesterol ester · low density lipoprotein cholesterol · phosphatidylcholine · phospholipid derivative · triacylglycerol · animal experiment · animal model · animal tissue · article · blood examination · controlled study · diet restriction · fatty liver · high performance thin layer chromatography · lipid blood level · lipid storage · liver homogenate · male · mass spectrometry · metabolomics · mouse · nonhuman · priority journal · reversed phase liquid chromatography · starvation · Animals · Blood · Cholesterol Esters · Chromatography, High Pressure Liquid · Disease Models, Animal · Fatty Liver · Food Deprivation · Lipoproteins, LDL · Liver · Male · Mice · Mice, Inbred C57BL · Phosphatidylcholines · Phospholipids · Triglycerides · Mammalia

Abstract

A metabolomic approach was applied to a mouse model of starvation-induced hepatic steatosis. After 24 h of fasting it appears that starvation reduced the phospholipids (PL), free cholesterol (FC), and cholesterol esters (CE) content of low-density lipoproteins (LDL). In liver lipid profiles major changes were observed using different techniques. High performance thin layer chromatography (HPTLC)-measurements of liver-homogenates indicated a significant rise of FC with 192%, triacylglycerols (TG) with 456% and cholesterol esters (CE) with 268% after 24 h of starvation in comparison with the control group. Reversed phase liquid chromatography coupled to mass spectrometry measurements (LC-MS) of liver homogenate indicated that the intensity of Phosphatidylcholine (PC) in the 24-h starvation group dropped to 90% of the value in the control group while the intensity of CE and TG increased to 157% and 331%, respectively, of the control group. Interestingly, a 49:4-TG with an odd number of C atoms appeared during starvation. This unique triacylglycerol has all characteristics of a biomarker for detection of hepatic steatosis. These observations indicate that in mammals liver lipid profiles are a dynamic system which are readily modulated by environmental factors like starvation. © 2007 Elsevier B.V. All rights reserved.