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Establishment of oxidative D-xylose metabolism in Pseudomonas putida S12

Author: Meijnen, J.P. · Winde, J.H.de · Ruijssenaars, H.J.
Type:article
Date:2009
Institution: TNO-Quality of Life, Business Unit Food and Biotechnology Innovations
Source:Applied and Environmental Microbiology, 9, 75, 2784-2791
Identifier: 241517
doi: doi:10.1128/AEM.02713-08
Keywords: Biology · Biomass yield · Catabolic pathway · Co-expression · D-Xylose · Dry weight · Glucose dehydrogenase · Gram-negative bacteria · Growth performance · Intracellular accumulation · Semialdehyde dehydrogenase · Sole carbon source · Biomass · Encoding (symbols) · Enzymes · Gene encoding · Glucose · Density measurement (specific gravity) · Caulobacter crescentus · Pseudomonas putida · Caulobacter vibrioides · Negibacteria · Pseudomonas putida · Aldehyde Oxidoreductases · Bacterial Proteins · Caulobacter crescentus · Gene Deletion · Genetic Engineering · Glucose 1-Dehydrogenase · Hydro-Lyases · Metabolic Networks and Pathways · Operon · Pseudomonas putida · Xylose

Abstract

The oxidative D-xylose catabolic pathway of Caulobacter crescentus, encoded by the xylXABCD operon, was expressed in the gram-negative bacterium Pseudomonas putida S12. This engineered transformant strain was able to grow on D-xylose as a sole carbon source with a biomass yield of 53% (based on g [dry weight] g D-xylose-1) and a maximum growth rate of 0.21 h -1. Remarkably, most of the genes of the xylXABCD operon appeared to be dispensable for growth on D-xylose. Only the xylD gene, encoding D-xylonate dehydratase, proved to be essential for establishing an oxidative D-xylose catabolic pathway in P. putida S12. The growth performance on D-xylose was, however, greatly improved by coexpression of xylXA, encoding 2-keto-3-deoxy-D-xylonate dehydratase and α-ketoglutaric semialdehyde dehydrogenase, respectively. The endogenous periplasmic glucose dehydrogenase (Gcd) of P. putida S12 was found to play a key role in efficient oxidative D-xylose utilization. Gcd activity not only contributes to D-xylose oxidation but also prevents the intracellular accumulation of toxic catabolic intermediates which delays or even eliminates growth on D-xylose. Copyright © 2009, American Society for Microbiology. All Rights Reserved. Chemicals / CAS: glucose dehydrogenase, 37250-49-0, 37250-50-3, 37250-84-3, 9028-53-9; hydrolyase, 9044-86-4; oxoglutarate dehydrogenase, 9031-02-1; xylose, 25990-60-7, 58-86-6; aldehyde dehydrogenase, 37353-37-0, 9028-86-8; 2,5-dioxovalerate dehydrogenase, 1.2.1.26; Aldehyde Oxidoreductases, 1.2.-; Bacterial Proteins; D-xylo-aldonate dehydratase, 4.2.1.-; Glucose 1-Dehydrogenase, 1.1.1.47; Hydro-Lyases, 4.2.1.-; Xylose