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Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study

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Author: Mestecky, J. · Hamilton, R.G. · Magnusson, C.G.M. · Jefferis, R. · Vaerman, J.P. · Goodall, M. · Lange, G.G. de · Moro, I. · Aucouturier, P. · Radl, J. · Cambiaso, C. · Silvain, C. · Preud'homme, J.L. · Kusama, K. · Carlone, G.M. · Biewenga, J. · Kobayashi, K. · Skvaril, F. · Reimer, C.B.
Institution: Gaubius Instituut TNO
Source:Journal of Immunological Methods, 2, 193, 103-148
Identifier: 233366
doi: doi:10.1016/0022-1759(95)00289-8
Keywords: Biology · (Human) · Am allotype · IgA · IgA subclass · Monoclonal antibody · Specificity · immunoglobulin a · immunoglobulin subclass · monoclonal antibody · antigen antibody reaction · article · controlled study · diagnostic value · enzyme immunoassay · human · immunoblotting · immunodiffusion · immunohistology · immunoreactivity · priority journal · serum · Animals · Antibodies, Monoclonal · Antibody Specificity · Binding Sites, Antibody · Epitopes · Humans · Immunoglobulin A · Immunoglobulin A, Secretory · Immunoglobulin Allotypes · Immunologic Techniques · Mice · Reference Standards · Secretory Component


51 monoclonal antibodies (McAb) with putative specificity for human IgA, the IgA subclasses, Am allotypes or secretory component (SC) were evaluated for immunoreactivity and specificity by nine laboratories employing immunodiffusion, agglutination, immunohistological assays, immunoblotting and direct binding and competitive inhibition enzyme immunoassays. McAbs specific for IgA PAN (n = 24), IgA1 (n = 7), IgA2 (n = 3), IgA2m(2) (n = 2), non-IgA2m(2) (n = 4) and SC or secretory IgA (n = 5) were identified that were immunoreactive and specific in the assays employed. The McAbs identified as IgA- or SC-reactive were shown to be non-reactive to human IgG, IgM, IgD, IgE, κ and λ by-direct binding and competitive inhibition immunoassays. Interestingly, no McAbs with restricted specificity for IgA2m(l) were identified. Some McAb displayed higher affinity and/or better performance in one or several of the assay groups. The IgA- and SC-specific McAbs identified in this international collaborative study have potential as immunochemical reference reagents to identify and quantitate monomeric and polymeric IgA in human serum and secretions. Chemicals/CAS: Antibodies, Monoclonal; Binding Sites, Antibody; Epitopes; Immunoglobulin A; Immunoglobulin A, Secretory; Immunoglobulin Allotypes; Secretory Component