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Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic

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Author: Noort, J.M. van · Berg, P. van den · Mattern, I.E.
Type:article
Date:1991
Institution: Medisch Biologisch Laboratorium TNO
Source:Analytical Biochemistry, 2, 198, 385-390
Identifier: 231618
Keywords: Bacitracin · Proteinase · Aspergillus niger · Chromatography · Nonhuman · Polyacrylamide gel electrophoresis · Priority journal · Protein analysis · Protein electrophoresis · Aspergillus niger · Bacitracin · Electrophoresis, Polyacrylamide Gel · Endopeptidases · Peptides · Protein Binding · Sepharose · Aspergillus niger · Fungi

Abstract

A method for the visualization of individual proteases within a complex biological sample is described. In a single chromatographic step, proteases can be separated from other biomolecules by selective binding to immobilized bacitracin, a peptide antibiotic. Following desorption, proteases may be separated by SDS-polyacrylamide gel electrophoresis. The application of this method is presented in the visualization of proteases secreted by the fungus Aspergillus niger.