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Complement-resistant Moraxella catarrhalis forms a genetically distinct lineage within the species

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Author: Verduin, C.M. · Kools-Sijmons, M. · Plas, J. van der · Vlooswijk, J. · Tromp, M. · Dijk, H. van · Banks, J. · verbrugh, H. · Belkum, A. van
Type:article
Date:2000
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Fems Microbiology Letters, 1, 184, 1-8
Identifier: 86992
doi: doi:10.1016/S0378-1097(00)00010-0
Keywords: Automated ribotyping · Complement resistance · Moraxella catarrhalis · Pulsed field gel electrophoresis · Bacterial genetics · Gene amplification · Moraxella catarrhalis · Nonhuman · Otitis media · Polymerase chain reaction · Priority journal · Random amplified polymorphic DNA · Restriction fragment length polymorphism · Bacterial Typing Techniques · Complement System Proteins · DNA, Bacterial · DNA, Ribosomal · Electrophoresis, Gel, Pulsed-Field · Genes, rRNA · Genotype · Moraxella (Branhamella) catarrhalis · Polymorphism, Restriction Fragment Length · Random Amplified Polymorphic DNA Technique · Bacteria (microorganisms) · Moraxella catarrhalis · Negibacteria · Prokaryota

Abstract

Moraxella catarrhalis is a bacterial species that has been implicated in 15^20% of all cases of otitis media in the USA and the complementresistantvariant of M. catarrhalis has been considered particularly pathogenic. A collection of geographically diverse, complement-sensitive (n = 28) and -resistant strains (n = 47) of M. catarrhalis was assembled in order to analyse the bacterial population structure. All strains were identified as M. catarrhalis by conventional microbiological and biochemical methods. Amplification of the small subunit (ssu) ribosomal RNA gene followed by restriction fragment length polymorphism (RFLP) analysis did not reveal consistent differences between serumsusceptible and -resistant M. catarrhalis isolates. Interestingly, upon automated ribotyping using the Qualicon RiboPrinter0 microbial characterisation system, the complement-sensitive and -resistant strains segregated into two groups. This suggested the existence of two clearly distinguishable lineages within the species M. catarrhalis. This observation was corroborated by pulsed field gel electrophoresis (PFGE) of DNA macro-restriction fragments, a non-ribosomal PCR RFLP procedure and random amplification of polymorphic DNA (RAPD) analysis. All procedures grouped the two variants similarly. Redefinition of the taxonomic status of complement-resistant M. catarrhalis or even the definition of a new species may be opportune. Chemicals/CAS: Complement System Proteins, 9007-36-7; DNA, Bacterial; DNA, Ribosomal