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Activation of toll-like receptors and dendritic cells by a broad range of bacterial molecules

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Author: Boele, L.C.L. · Bajramovic, J.J. · Vries, A.M.M.B.C. de · Voskamp-Visser, I.A.I. · Kaman, W.E. · Kleij, D. van der
Institution: TNO Defensie en Veiligheid
Source:Cellular Immunology, 1-2, 255, 17-25
Identifier: 27880
doi: doi:doi:10.1016/j.cellimm.2008.09.002
Keywords: Biology · Bacteria · Cytokines · Dendritic cell · Pathogens · Toll-like receptor · toll like receptor · toll like receptor 2 · toll like receptor 4 · toll like receptor 5 · article · cell maturation · cell polarity · controlled study · cytokine production · dendritic cell · genetic transfection · human · human cell · priority journal · Staphylococcus aureus · T lymphocyte · Vibrio cholerae · Yersinia pestis · Yersinia pseudotuberculosis · Animals · Antigens, CD · Bacteria · Biological Markers · Cell Line · Coculture Techniques · Cytokines · Dendritic Cells · HLA-DR Antigens · Humans · Ligands · Protein Isoforms · T-Lymphocytes · Toll-Like Receptors · Ultraviolet Rays · Bacteria (microorganisms) · Negibacteria · Posibacteria


Activation of pattern recognition receptors such as Toll-like receptors (TLRs) by pathogens leads to activation and maturation of dendritic cells (DC), which orchestrate the development of the adaptive immune response. To create an overview of the effects of a broad range of pathogenic bacteria, their capacity to activate TLRs and to affect DC maturation, cytokine production and T cell polarizing capacity were determined. Different bacterial species differed in their potency to affect these parameters. In general, on the DC level differences were found in the maturation-inducing capacity of gram-negative and gram-positive bacteria. Remarkably, these differences did not result in differential polarization of the T cell response. With respect to TLRs, TLR4 activation by pathogens correlated with their ability to induce DC maturation, while for TLR2 and TLR5 such a correlation was absent. Taken together, this study provides insight into qualitative differences and general effects of pathogen-derived molecules on dendritic cells. © 2008 Elsevier Inc. All rights reserved. Chemicals / CAS: toll like receptor 2, 203811-81-8; toll like receptor 4, 203811-83-0; toll like receptor, 409141-78-2; Antigens, CD; Biological Markers; Cytokines; HLA-DR Antigens; Ligands; Protein Isoforms; Toll-Like Receptors