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Relevance of microbial interactions to predictive microbiology

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Author: Malakar, P.K. · Barker, G.C. · Zwietering, M.H. · Riet, K. van 't
Type:article
Date:2003
Institution: TNO Voeding Centraal Instituut voor Voedingsonderzoek TNO
Source:International Journal of Food Microbiology, 84, 263-272
Identifier: 57799
doi: doi:10.1016/S0168-1605(02)00424-5
Keywords: Nutrition Biology · Food technology · Broth cultures · Colony growth · Intra-colony interactions · Microbial interactions · Predictive microbiology · Analysis · Bacterial growth · Bacterium colony · Bacterium culture · Controlled study · Density · Diffusion · Fermented product · Food spoilage · Growth inhibition · Inoculation · Listeria monocytogenes · Model · Nonhuman · Prediction · Bacterial count · Biological model · Culture medium · Food control · Growth, development and aging · Population density · Prediction and forecasting · Bacteria (microorganisms) · Lactobacillus · Lactobacillus curvatus · Listeria · Colony Count, Microbial · Culture Media · Food Microbiology · Models, Biological · Population Density · Predictive Value of Tests

Abstract

Microbial interaction can be ignored in predictive microbiology under most conditions. We show that interactions are only important at high population densities, using published data on inhibition of growth of Listeria monocytogenes in broth. Our analysis using growth models from predictive microbiology indicated that interactions only occur at population densities of ∼108 cfu/ml of the protective cultures. Spoilage is evident at these levels, except for fermented foods. In bacterial colonies, diffusion limitation acts as a constraint to growth. We have shown that these constraints only become important after large outgrowth of colonies (in the order of 5-log growth in Lactobacillus curvatus colonies), which depends on the initial inoculation density. Intra-colony interactions play an important role under these conditions. There is no large outgrowth of colonies when the initial inoculation densities are high and broth culture growth can be used to approximate colony growth. © 2002 Elsevier Science B.V. All rights reserved. Chemicals/CAS: Culture Media