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Protein detection on biotin-derivatized polyallylamine by optical microring resonators

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Author: Ullien, D. · Harmsma, P.J. · Chakkalakkal Abdulla, S.M.C. · Boer, B.M. de · Bosma, D. · Sudhölter, E.J.R. · Smet, L.C.P.M. de · Jager, W.F.
Type:article
Date:2014
Publisher: Optical Society of American (OSA)
Source:Optics Express, 13, 22, 16585-16594
Identifier: 513442
Keywords: Electronics · Optical resonators · Biomolecular interactions · Biosensing platforms · Highly sensitive detections · Microring resonance · Optical microring resonator · Polyallylamine · Protein detection · Sensitive devices · Coenzymes · High Tech Systems & Materials · Industrial Innovation · Physics & Electronics · OPT - Optics · TS - Technical Sciences

Abstract

Silicon optical microring resonators (MRRs) are sensitive devices that can be used for biosensing. We present a novel biosensing platform based on the application of polyelectrolyte (PE) layers on such MRRs. The top PE layer was covalently labeled with biotin to ensure binding sites for antibodies via a streptavidin-biotin binding scheme. Monitoring the shift in the microring resonance wavelength allows realtime, highly sensitive detection of the biomolecular interaction. © 2014 Optical Society of America.