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Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry

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Author: Balvers, M.G.J. · Verhoeckx, K.C.M. · Witkamp, R.F.
Type:article
Date:2009
Institution: TNO Kwaliteit van Leven
Source:Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 14-15, 877, 1583-1590
Identifier: 241537
doi: doi:10.1016/j.jchromb.2009.04.010
Keywords: Biology · Analytical research · Biomedical research · Endocannabinoids · Human plasma · LC-MS/MS · Sample pre-treatment · Endocannabinoids · Gradient elutions · Human plasma · LC-MS/MS · Liquid chromatography-tandem mass spectrometries · Quadrupole · Quantitative methods · Related compounds · Sample pre-treatment · Solid-phase extractions · Acetonitrile · Brain · Chromatographic analysis · Ethers · Glycerol · High performance liquid chromatography · Mass spectrometry · Phase separation · Plasma (human) · Plasmas · Extraction · 2 arachidonoylglycerol · 2 arachidonylglycerol ether · anandamide · dihomo gamma linolenoylethanolamide · docosatetraenoylethanolamide · dopamine derivative · endocannabinoid · ethanolamine · glycine derivative · n arachidonoylglycine · n arachidonyldopamine · n oleoyl dopamine · n oleoylethanolamine · o arachidonoylethanolamide · octadecylamine · palmidrol · stearoylethanolamide · unclassified drug · article · diagnostic accuracy · drug determination · drug isolation · human · human cell · liquid chromatography · priority journal · quantitative analysis · sensitivity and specificity · solid phase extraction · tandem mass spectrometry · validation process · Chromatography, Liquid · Endocannabinoids · Humans · Tandem Mass Spectrometry

Abstract

A sensitive and specific LC-MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-γ-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 μg/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels. © 2009 Elsevier B.V. All rights reserved.