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RPLC-lon-trap-FTMS method for lipid profiling of plasma: Method validation And application to p53 mutant mouse model

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Author: Hu, C. · Dommelen, J. van · Heijden, R. van der · Spijksma, G. · Reijmers, T.H. · Wang, M. · Slee, E. · Lu, X. · Xu, G. · Greef, J. van der · Hankemeier, T.
Type:article
Date:2008
Institution: TNO Kwaliteit van Leven
Source:Journal of Proteome Research, 11, 7, 4982-4991
Identifier: 280031
doi: doi:10.1021/pr800373m
Keywords: Analytical research · FTMS · Lipid profiling · Lipidomics · Metabolite profiling · Metabolomics · P53 mouse model · Protein p53 · Lipid · Article · Controlled study · High performance liquid chromatography · Human · Ion cyclotron resonance mass spectrometry · Ion trap mass spectrometry · Lipid analysis · Lipid blood level · Mouse · Mutational analysis · Nonhuman · Phenotype · Priority journal · Protein expression · Reversed phase liquid chromatography · Animal · Blood · Cyclotron · Infrared spectroscopy · Liquid chromatography · Methodology · Mouse mutant · Reproducibility · Tandem mass spectrometry · Mus · Animals · Chromatography, Liquid · Cyclotrons · Lipids · Mice · Mice, Mutant Strains · Reproducibility of Results · Spectroscopy, Fourier Transform Infrared · Tandem Mass Spectrometry · Tumor Suppressor Protein p53

Abstract

A reversed-phase liquid chromatography-linear ion trap-Fourier transform ion cyclotron resonance-mass spectrometry method was developed for the profiling of lipids in human and mouse plasma. With the use of a fused-core C<sub>8</sub> column and a binary gradient, more than 160 lipids belonging to eight different classes were detected in a single LC-MS run. The method was fully validated and the analytical characteristics such as linearity (R<sup>2</sup>, 0.994-1.000), limit of detection (0.08-1.28 μ/mL plasma), repeatability (RSD, 2.7-7.9%) and intermediate precision (RSD, 2.7-15.6%) were satisfactory-The method was successfully applied to p53 mutant mice plasma for studying some phenotypic effects of p53 expression. © 2008 American Chemical Society.