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Extracorporeal irradiation of the blood in a rat model for human acute myelocytic leukemia. Increased efficacy after combination with cell mobilization by low-molecular-weight dextran sulfate

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Author: Hagenbeek, A. · Martens, A.C.M.
Type:article
Date:1981
Institution: Radiobiologisch Instituut TNO
Source:Radiation Research, 1, 88, 144-154
Identifier: 229046
Keywords: Biology · chromium 51 · Acute myeloblastic leukemia · Animal experiment · Biological model · Blood · Blood and hemopoietic system · Blood extracorporeal radiation · Irradiation · Animal · Blood · Cell Compartmentation · Dextran Sulfate · Dextrans · Human · Leukemia, Experimental · Leukemia, Myelocytic, Acute · Male · Rats · Rats, Inbred BN

Abstract

The efficacy of extracorporeal irradiation of the blood (ECIB) in combination with cell mobilization by dextran sulfate (DS; MW 17,000) was investigated in a rat model for human acute myelocytic leukemia. Repeated injections with DS (q 3 hr) induced a significant increase in the number of peripheral leukemic cells, i.e., up to 4.5 times the original number 6 hr after the first injection. Cell mobilization in combination with ECIB (2 x 8 hr) caused a depletion of the blood compartments and the rapidly exchangeable tissue pool down to 10-25% of their original sizes, as determined by measuring the distribution of infused 51Cr-labeled leukemic cells and organ weights. These size reductions are about two times as great as those in rats treated with ECIB alone. In addition, the slowly exchangeable tissue pools are significantly depleted when DS is added to ECIB treatment. The reduction in the total tumor load was about 50%. This, however, is too small to result in a significant difference in survival time between treated and nontreated leukemic rats. Chemicals/CAS: chromium 51, 14392-02-0; dextran sulfate, 9011-18-1, 9042-14-2; Dextran Sulfate, 9042-14-2; Dextrans, 9004-54-0