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The effect of protein-protein and protein-membrane interactions on membrane fouling in ultrafiltration

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Author: Huisman, I.H. · Prádanos, P. · Hernández, A.
Type:article
Date:2000
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Journal of Membrane Science, 1-2, 179, 79-90
Identifier: 56898
doi: doi:10.1016/S0376-7388(00)00501-9
Keywords: Nutrition · Fouling · Physicochemical interactions · Protein · Protein-membrane interactions · Ultrafiltration · Atomic force microscopy · Mechanical permeability · PH effects · Proteins · Ultrafiltration · Bovine serum albumin · Polymeric membranes · Bovine serum albumin · Electrolyte · Polymer · Polysulfone · Protein · Membrane · Membrane technology · Ultrafiltration · Artificial membrane · Atomic force microscopy · Membrane permeability · Membrane transport · Performance · PH · Priority journal · Protein analysis · Protein protein interaction · Protein transport · Ultrafiltration

Abstract

It was studied how protein-protein and protein-membrane interactions influence the filtration performance during the ultrafiltration of protein solutions over polymeric membranes. This was done by measuring flux, streaming potential, and protein transmission during filtration of bovine serum albumin (BSA) solutions at various pH values using various membranes with different cut-off values. It was found that protein-membrane interactions influence the fouling behaviour in the initial stages of filtration. In the high-fouling regime (later stages of filtration), protein-protein interactions dictate the overall performance. AFM images of the membrane surfaces taken after the filtration experiments showed that the membranes were totally covered by a protein fouling layer. The structure of this fouling layer depended strongly on pH. In particular, very open structures with high permeabilities were found at low pH (below the iso-electric point of the protein). These induced high values of flux and protein transmission. (C) 2000 Elsevier Science B.V. It was studied how protein-protein and protein-membrane interactions influence the filtration performance during the ultrafiltration of protein solutions over polymeric membranes. This was done by measuring flux, streaming potential, and protein transmission during filtration of bovine serum albumin (BSA) solutions at various pH values using various membranes with different cut-off values. It was found that protein-membrane interactions influence the fouling behaviour in the initial stages of filtration. In the high-fouling regime (later stages of filtration), protein-protein interactions dictate the overall performance. AFM images of the membrane surfaces taken after the filtration experiments showed that the membranes were totally covered by a protein fouling layer. The structure of this fouling layer depended strongly on pH. In particular, very open structures with high permeabilities were found at low pH (below the iso-electric point of the protein). These induced high values of flux and protein transmission.