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Detection of sulfur mustard adducts in human callus by phage antibodies

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Author: Bikker, F.J. · Mars-Groenendijk, R.H. · Noort, D. · Fidder, A. · Schans, G.P. van der
Type:article
Date:2007
Institution: TNO Defensie en Veiligheid
Source:Chemical Biology and Drug Design, 5, 69, 314-320
Identifier: 239957
doi: doi:10.1111/j.1747-0285.2007.00504.x
Keywords: Immunodiagnostics · Phage display · Post translational modifications · Chemical warfare agent · Keratin · Keratin antibody · Mustard gas · Antibody affinity · Article · Bacteriophage · Bacterium culture · Callus · Culture technique · Evaluation research · Exposure · Gene library · Human · Human tissue · Hybridoma · Nonhuman · Nucleotide sequence · Phage display · Priority journal · Program development · Protein isolation · Research · Serodiagnosis · Skin · Antibodies, Monoclonal · Bacteriophages · Base Sequence · DNA Primers · Humans · Mustard Gas · Molecular Sequence Numbers: GENBANK: DQ184510, DQ184511, DQ184512, DQ184513; · Chemicals / CAS: mustard gas, 505-60-2; Antibodies, Monoclonal; DNA Primers; Mustard Gas, 505-60-2

Abstract

As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time-consuming hybridoma technique. The Tomlinson I and J phage libraries were used to select phage antibodies exhibiting affinity for sulfur mustard adducts on keratins, isolated from human callus. Two kinds of phage antibodies were obtained: antibodies recognizing keratin and antibodies recognizing keratin which was exposed to sulfur mustard. These phage antibodies retained activity after repeated culturing and culturing in larger volumes. For the first time antibody phage display was successfully applied for immunodiagnostics of a chemical warfare agent. © 2007 The Authors.