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Multispectral characterization of tissues encountered during laparoscopic colorectal surgery

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Author: Schols, R.M. · Dunias, P. · Wieringa, F.P. · Stassen, L.P.
Type:article
Date:2013
Source:Medical Engineering and Physics, 7, 35, 1044-1050
Identifier: 485636
doi: doi:10.1016/j.medengphy.2013.01.004
Keywords: Health · Automated tissue recognition · Iatrogenic injury prevention · Laparoscopic colorectal surgery · Spectral analysis · Healthy for Life · Healthy Living · Mechatronics, Mechanics & Materials · EAM - Equipment for Additive Manufacturing · TS - Technical Sciences

Abstract

Aims: This study investigated the feasibility of automated differentiation between essential tissue types encountered during laparoscopic colorectal surgery using spectral analysis. Methods: Wide band (440-1830 nm) spectra were collected using an optical fiber probe and spectrometer from freshly explanted, ex vivo, human colonic specimens. These data were normalized at 810 nm (an isobestic wavelength for hemoglobin and oxy-hemoglobin) and mathematically analyzed using total principal component regression (TPCR). Results: 929 spectra were collected from specimens of 19 patients, distinguishing 5 tissue types: mesenteric fat (MF, n = 269), blood vessels (BV, n = 377), colonic tissue (CT, n = 213), ureter (UR, n = 10) and tumorous tissue in colon (TT, n = 60). For each individual tissue type the distinctive ability was determined against all other tissue types pooled as a group. Paired probability density function (PDF) of "tissue" (centered around label 1) versus "all other pooled tissues" (centered around label 0) and the cumulative distribution function (CDF) at label crossover value 0.5 was determined for each tissue type (MF: CDF = 0.99 [SD = 0.19]; BV: CDF = 0.95 [SD = 0.29]; CT: CDF = 0.98 [SD = 0.22]; UR: CDF = 0.99 [SD = 0.09]; TT: CDF = 0.99 [SD = 0.18]). Conclusion: Automated spectral differentiation of blood vessel, ureter, mesenteric adipose tissue, colonic tissue and tumorous tissue in colon, is feasible in freshly explanted human colonic specimens. These results may be exploited for further steps toward multi- or hyperspectrally enhanced in vivo (laparoscopic) surgical imaging. © 2013 IPEM. Published by Elsevier Ltd. All rights reserved.