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Embryotoxicity and teratogenicity study with α-cyclodextrin in rabbits

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Author: Waalkens-Berendsen, D.H. · Smits-Van Prooije, A.E. · Bär, A.
Type:article
Date:2004
Institution: TNO Voeding
Source:Regulatory Toxicology and Pharmacology, SUPPL., 39
Identifier: 237811
doi: doi:10.1016/j.yrtph.2004.05.003
Keywords: Biology · Toxicology and Applied Pharmacology · Cyclodextrin · Dietary Fiber · Lactose · Reproductive toxicity · alpha cyclodextrin · animal experiment · animal model · animal tissue · article · artificial insemination · body weight · chemical teratogenesis · controlled study · corpus luteum · diarrhea · embryotoxicity · female · fetus weight · food intake · gestational age · male · nidation · nonhuman · placenta weight · priority journal · sex ratio · sexual behavior · species comparison · teratogenicity · uterus weight · weight reduction · Abnormalities, Drug-Induced · Administration, Oral · alpha-Cyclodextrins · Animals · Cyclodextrins · Embryonic and Fetal Development · Female · Male · Pregnancy · Prenatal Exposure Delayed Effects · Rabbits · Animalia · Oryctolagus cuniculus

Abstract

In a standard embryotoxicity/teratogenicity study, α-cyclodextrin (α-CD) was administered to groups of sixteen, artificially inseminated New Zealand White rabbits at dietary concentrations of 0, 5, 10, or 20%. An additional group received a diet containing 20% lactose. Treatment started on day 0 of gestation and ended on day 29 when the animals were killed. Except for the occurrence of transient diarrhoea in one rabbit of the 20% α-CD group for a few days, the treatment was well tolerated. A reduced food intake in the 20% α-CD group during the first week of treatment resulted in a reduced weight gain from day 0 to 12 of the study. However, the difference to the controls was not significant and at termination of the study body weights were similar in all groups. Even at the highest dose level, which corresponds to an intake of 5.9-7.5g/kg bw/day, no signs of maternal toxicity were observed. Maternal reproductive performance was not affected by the treatment. Uterine weight, placental weight, fetal weight, number of fetuses, sex ratio, number of implanation sites, resorptions, and corpora lutea did not differ among the groups. Visceral and skeletal examinations of the fetuses did not reveal any malformations, anomalies or variations that could be attributed to treatment. It was concluded that dietary α-CD is generally well tolerated by pregnant rabbits, has no adverse effect on maternal reproductive performance and is not embryotoxic, fetotoxic, or teratogenic at dietary concentrations of up to 20%, the highest dose level tested. © 2004 Elsevier Inc. All rights reserved.