Repository hosted by TU Delft Library

Home · Contact · About · Disclaimer ·
 

To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

Publication files not online:

Author: Aguirre, M. · Ramiro-Garcia, J. · Koenen, M.E. · Venema, K.
Type:article
Date:2014
Source:Journal of Microbiological Methods, 107, 1-7
Identifier: 517662
doi: doi:10.1016/j.mimet.2014.08.022
Keywords: Biology · Fecal inoculum preparation · Fermentation · Gut microbiota · In vitro model · Pooling · Branched chain fatty acid · Fatty acid · Isobutyric acid · Isovaleric acid · Short chain fatty acid · Unclassified drug · Actinobacteria · Adult · Bacteroidetes · Clostridium · Controlled study · Feces · Female · Fermentation technique · Firmicutes · Genus · Human · Human experiment · In vitro study · Inoculation · Intestine flora · Microbial community · Nonhuman · Normal human · Prevotella · Proteobacteria · Sample · Turicibacter · Verrucomicrobia · Biomedical Innovation · Healthy Living · Life · MSB - Microbiology and Systems Biology · ELSS - Earth, Life and Social Sciences

Abstract

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments. Chemicals/CAS: isobutyric acid, 5711-69-3, 79-31-2; isovaleric acid, 503-74-2, 539-66-2