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Anaerobic conversion of lactic acid to acetic acid and 1,2-propanediol by Lactobacillus buchneri

Author: Oude Elferink, S.J.W.H. · Krooneman, J. · Gottschal, J.C. · Spoelstra, S.F. · Faber, F. · Driehuis, F.
Type:article
Date:2001
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Applied and Environmental Microbiology, 1, 67, 125-132
Identifier: 72121
doi: doi:10.1128/AEM.67.1.125-132.2001
Keywords: Environment · Acetic Acid · Anaerobiosis · Biodegradation, Environmental · Culture Media · Hydrogen-Ion Concentration · Lactic Acid · Lactobacillus · NADH Dehydrogenase · Propylene Glycol · Temperature · Kefir · Lactobacillus buchneri · Lactobacillus hilgardii · Lactobacillus kefiri · Lactobacillus parabuchneri · Posibacteria

Abstract

The degradation of lactic acid under anoxic conditions was studied in several strains of Lactobacillus buchneri and in close relatives such as Lactobacillus parabuchneri, Lactobacillus kefir, and Lactobacillus hilgardii. Of these lactobacilli, L. buchneri and L. parabuchneri were able to degrade lactic acid under anoxic conditions, without requiring an external electron acceptor. Each mole of lactic acid was converted into approximately 0.5 mol of acetic acid, 0.5 mol of 1,2-propanediol, and traces of ethanol. Based on stoichiometry studies and the high levels of NAD-linked 1,2-propanediol-dependent oxidoreductase (530 to 790 nmol min-1 mg of protein-1), a novel pathway for anaerobic lactic acid degradation is proposed. The anaerobic degradation of lactic acid by L. buchneri does not support cell growth and is pH dependent. Acidic conditions are needed to induce the lactic-acid-degrading capacity of the cells and to maintain the lactic-acid-degrading activity. At a pH above 5.8 hardly any lactic acid degradation was observed. The exact function of anaerobic lactic acid degradation by L. buchneri is not certain, but some results indicate that it plays a role in maintaining cell viability. Chemicals/CAS: acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; alcohol, 64-17-5; lactic acid, 113-21-3, 50-21-5; nicotinamide adenine dinucleotide, 53-84-9; oxidoreductase, 9035-73-8, 9035-82-9, 9037-80-3, 9055-15-6; propylene glycol, 57-55-6; Acetic Acid, 64-19-7; Culture Media; Lactic Acid, 50-21-5; NADH Dehydrogenase, EC 1.6.99.3; Propylene Glycol, 57-55-6