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Generic solid phase extraction-liquid chromatography-tandem mass spectrometry method for fast determination of drugs in biological fluids

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Author: Schellen, A. · Ooms, B. · Lagemaat, D. van de · Vreeken, R. · Dongen, W.D. van
Type:article
Date:2003
Institution: TNO Voeding
Source:Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2, 788, 251-259
Identifier: 237091
doi: doi:10.1016/S1570-0232(02)01013-9
Keywords: Drugs Pharmacology · Analytical research · Drug analysis · Generic method · Body fluids · Drug products · Mass spectrometers · Solid phase extraction · High performance liquid chromatography · caffeine · carbamazepine · paclitaxel · paracetamol · procainamide · propranolol · ranitidine · sulfadiazine · sulfamerazine · theobromine · theophylline · accuracy · article · controlled study · drug blood level · drug determination · high performance liquid chromatography · human · linear system · physical chemistry · priority journal · quantitative analysis · solid phase extraction · tandem mass spectrometry · technique · time · Chromatography, High Pressure Liquid · Humans · Mass Spectrometry · Pharmaceutical Preparations · Reproducibility of Results · Sensitivity and Specificity · Spectrophotometry, Ultraviolet

Abstract

A generic method was developed for the fast determination of a wide range of drugs in serum or plasma. The methodology comprises generic solid-phase extraction, on-line coupled to gradient HPLC with tandem mass spectrometric detection (SPE-LC-MS/MS). The individual components of the SPE-LC-MS/MS system were optimized in an integrated approach to maximize the application range and minimize the method development time. The optimized generic SPE-LC-MS/MS protocol was evaluated for 11 drugs with different physicochemical properties. Good quantification for 10 out of 11 of the pharmaceuticals in serum or plasma could be readily achieved. The quantitative assays gave recoveries better than 95%, lower quantification limits of 0.2-2.0 ng/ml, acceptable precision and accuracy and good linearity over 2-4 orders of magnitude. Carry-over was determined to be in the range of 0.02-0.10%, without optimization. © 2003 Elsevier Science B.V. All rights reserved. Chemicals/CAS: caffeine, 30388-07-9, 58-08-2; carbamazepine, 298-46-4, 8047-84-5; paclitaxel, 33069-62-4; paracetamol, 103-90-2; procainamide, 51-06-9, 614-39-1; propranolol, 13013-17-7, 318-98-9, 3506-09-0, 4199-09-1, 525-66-6; ranitidine, 66357-35-5, 66357-59-3; sulfadiazine, 547-32-0, 68-35-9; sulfamerazine, 127-58-2, 127-79-7; theobromine, 83-67-0; theophylline, 58-55-9, 5967-84-0, 8055-07-0, 8061-56-1, 99007-19-9; Pharmaceutical Preparations