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Extrapulmonary colony formation after intravenous injection of tumour cells into heparin treated animals

Author: Maat, B.
Institution: Radiobiologisch Instituut TNO
Source:British Journal of Cancer, 3, 37, 369-376
Identifier: 228476
Keywords: fibrin · animal experiment · blood and hemopoietic system · blood clotting · cancer graft · colony formation · dose response · drug comparison · drug response · intraperitoneal drug administration · intravenous drug administration · lung · metastasis · mouse · respiratory system · theoretical study · Animal · Cell Count · Heparin · Lung Neoplasms · Mice · Neoplasm Circulating Cells · Neoplasm Metastasis · Neoplasms, Experimental · Rats · Thoracic Neoplasms


Recent data on extrapulmonary colony formation after heparin administration are inconclusive. A systemic study of this topic was undertaken with 4 experimental tumour systems and 2 distinct periods of reduced clotting capacity in rats and mice. I.v. injection of various numbers of tumour cells into i.p. heparinized animals leads to: Significant reduction in the number of lung colonies. The effect after 9 h anticoagulation is equal to or probably greater than after 2 h. The reduction in the number of lung colonies caused by heparin is independent of the number of cells injected. The number of extrapulmonary extrathoracic colonies is significantly increased by heparin in 3 of the 4 tumour systems. The number of extrapulmonary intrathoracic colonies is probably unaffected. The increase in extrapulmonary extrathoracic colony formation is not related to the degree of reduction in lung colonies. These data lead to the conclusion that the capacity of the lung capillaries to trap tumour cells can be decreased by heparin-induced alterations in fibrin formation. This results in a lodgement of tumour cells throughout the body which is far more pronounced than in animals with normal clotting capacity. Chemicals/CAS: fibrin, 9001-31-4; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; Heparin, 9005-49-6