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Rapid and generic identification of respiratory viruses with mass spectrometry - Poster

Author: Majchrzykiewicz-Koehorst, J.A. · Heikens, E. · Trip, H. · Hulst, A.G. · Jong, A.L. de · Viveen, M.C. · Sedee, N.J.A. · Plas, J. van der · Coenjaerts, F.E.J. · Paauw, A.
Source:NVMM 2014, Arnhem, Nederland, 15-16 april 2014
Identifier: 531255
Keywords: Biology · Identification · Influenza virus · LC-MS/MS · MALDI-TOF MS · Mass spectrometry · Virological diagnosis · virus protein · Amino acid sequence · Controlled study · Influenza virus A · Intermethod comparison · Liquid chromatography · Matrix assisted laser desorption ionization time of flight mass spectrometry · Metapneumovirus · Nonhuman · Nucleotide sequence · Process development · Reliability · Respiratory syncytial pneumovirus · Respiratory virus · RNA sequence · Sensitivity analysis · Sequence analysis · Tandem mass spectrometry · Virus identification · Virus strain · Bacteria (microorganisms) · Human metapneumovirus · Influenza A virus · Orthomyxoviridae · Respiratory syncytial virus · Life · CBRN - CBRN Protection · TS - Technical Sciences


Tested respiratory viruses: influenza A virus, respiratory syncytial virus (RSV) and human metapneumovirus (hMPV), were successfully identified directly from crude cell cultures using liquid chromatography-tandem MS (LC-MS/MS). The identification was preceded with the simple, generic and fast sample preparation method. The determined identification limit was only one order of magnitude higher than required for identification from a clinical specimen. Because identification is based on amino acid sequences deposited in GenBank, other viruses are likely to be identified using the developed method. Further improvement of the sample preparation method, may soon bridge the gap between research and clinical diagnostics. We conclude that MS-based techniques have the potential to become a complementary method to PCR in viral diagnostics.