To study the effects of a tactile feedback signal in a computer mouse on reduction of hovering behaviour and consequently on changes in muscle load, productivity, comfort and user friendliness, a comparative, experimental study with repeated measures was conducted. Fifteen subjects performed five trials with different mouse actions and a standardised task, once with a mouse with the feedback signal and once with a mouse without the feedback signal. Holding the hand just above the mouse caused higher muscle loading than clicking and scrolling. Holding the hand on the mouse caused higher muscle loading than resting the hand on the desk. The feedback signal effectively decreased hovering behaviour. It also led to a more dynamic activation pattern of the extensor muscles of the forearm. The overall opinion of the feedback signal for future use was rated as somewhat variable. No effects on discomfort or productivity were found. The use of a mouse with a tactile vibrating feedback signal seems promising for preventing arm complaints, although more research is needed to establish the clinical relevance.