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Medium optimization for nuclease P1 production by Penicillium citrinum in solid-state fermentation using polyurethane foam as inert carrier

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Author: Zhu, Y. · Knol, W. · Smits, J.P. · Bol, J.
Type:article
Date:1996
Institution: TNO Voeding
Source:Enzyme and Microbial Technology, 2, 18, 108-112
Identifier: 233207
doi: doi:10.1016/0141-0229(95)00082-8
Keywords: Nutrition · Inert carrier · Medium optimization · Nuclease P1 · Penicillium citrinum · Polyurethane foam · Solid-state fermentation · Biotechnology · Fermentation · Foamed plastics · Microorganisms · Optimization · Polyurethanes · Fractional factorial experimental design · Inert carrier · Nuclease P1 · Penicillium citrinum · Polyurethane foam · Solid state fermentation · Wheat bran · Enzymes · Nuclease · Polyurethan foam · Article · Culture medium · Enzyme activity · Enzyme synthesis · Fermentation · Methodology · Nonhuman · Penicillium · Wheat bran

Abstract

A solid-state fermentation system, using polyurethane foam as an inert carrier, was used for the production of nuclease P1 by Penicillium citrinum. Optimization of nuclease P1 production was carried out using a synthetic liquid medium. After a two-step medium optimization using a fractional factorial experimental design, nuclease P1 productivity and specific activity are greatly increased. Nuclease P1 productivity and specific activity are ninefold and fourfold higher, respectively, than in solid-state fermentation of wheat bran and 500-fold and 20-fold higher, respectively, than in submerged fermentation. Additional advantages of the solid-state fermentation process using inert carrier are discussed. A solid-state fermentation system, using polyurethane foam as an inert carrier, was used for the production of nuclease P1 by Penicillium citrinum. Optimization of nuclease P1 production was carried out using a synthetic liquid medium. After a two-step medium optimization using a fractional factorial experimental design, nuclease P1 productivity and specific activity are greatly increased. Nuclease P1 productivity and specific activity are ninefold and fourfold higher, respectively, than in solid-state fermentation of wheat bran and 500-fold and 20-fold higher, respectively, than in submerged fermentation. Additional advantages of the solid-state fermentation process using inert carrier are discussed.