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IFN-beta modulates specific T cell responses in vitro but does not affect Experimental Autoimmune Encephalomyelitis in the SJL mouse

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Author: Luca, M.E. · Visser, L. · Lucas, C.J. · Nagelkerken, L.
Institution: TNO Preventie en Gezondheid
Source:Journal of Neuroimmunology, 1-2, 100, 190-196
Identifier: 235305
doi: doi:10.1016/S0165-5728(99)00198-8
Keywords: Biology · EAE · Interferon-β · Multiple sclerosis · Murine · T lymphocyte · Animals · Cells, Cultured · Dose-Response Relationship, Drug · Encephalomyelitis, Autoimmune, Experimental · Enzyme-Linked Immunosorbent Assay · Female · Interferon-beta · Interleukin-10 · Lymphocytes · Mice · Mice, Inbred Strains · Pertussis Toxin · Recombinant Proteins · Th2 Cells · Time Factors · Virulence Factors, Bordetella


In this study, mouse recombinant IFN-β was shown to favor PLP139-151-specific Th2 responses in vitro, by inhibiting IFN-γ production and stimulating IL-4 and IL-10 production. IFN-β (5000 U/day) failed to prevent the development or severity of EAE induced with PLP139-151. Whereas efficacy of IL-10 was found in the B. pertussis assisted but not in the pertussigen-assisted EAE model, both models appeared insensitive to IFN-β. Also the combination of (suboptimal) IL-10 and IFN-β appeared ineffective in inhibiting disease. However, the PLP139-151-specific IL-10 production by T cells from these mice appeared significantly more sensitive to the stimulatory effect of IFN-β in vitro. It is concluded that despite its Th2 promoting effects, IFN-β is not effective in inhibiting EAE in this study. Copyright (C) 1999 Elsevier Science B.V. Chemicals/CAS: Interferon-beta, 77238-31-4; Interleukin-10, 130068-27-8; Pertussis Toxin, EC; Recombinant Proteins; Virulence Factors, Bordetella