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Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori

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Author: Lokman, B.C. · Joosten, V. · Hovenkamp, J. · Gouka, R.J. · Verrips, C.T. · Hondel, C.A.M.J.J. van den
Type:article
Date:2003
Source:Journal of Biotechnology, 2, 103, 183-190
Identifier: 237151
doi: doi:10.1016/S0168-1656(03)00109-3
Keywords: Biotechnology · Aspergillus awamori · Heme limitation · Heterologous protein production · Peroxidase · Cell culture · Enzyme kinetics · Fungi · Growth kinetics · Molecular weight · Western blot analysis · Biotechnology · fungal enzyme · peroxidase · xylan endo 1,3 beta xylosidase · arthromyces ramosus · article · Aspergillus awamori · culture medium · enzyme analysis · enzyme synthesis · fungus · molecular weight · nonhuman · priority journal · Western blotting · Aspergillus · Biotechnology · Blotting, Northern · Blotting, Southern · Fermentation · Heme · Mitosporic Fungi · Peroxidase · Plasmids · Promoter Regions (Genetics) · 'Arthromyces ramosus' · Aspergillus · Aspergillus awamori · Fungi

Abstract

The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l-1 in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP. © 2003 Elsevier Science B.V. All rights reserved.