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Modulation of dietary fat-enhanced colorectal carcinogenesis in N-methyl-N'-nitro-N-nitrosoguanidine-treated rats by a vegetables-fruit mixture

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Author: Rijnkels, J.M. · Hollanders, V.H.M. · Woutersen, R.A. · Koeman, J.H. · Alink, G.M.
Institution: TNO Voeding
Source:Nutrition and Cancer, 1, 29, 90-95
Identifier: 85337
Keywords: Nutrition · Administration, Rectal · Animals · Carcinogens · Colorectal Neoplasms · Dietary Fats · Fruit · Male · Methylnitronitrosoguanidine · Rats · Rats, Wistar · Vegetables · Animalia


The modulation of a vegetables-fruit mixture on N-methyl-N'-nitro-N- nitrosoguanidine (MNNG)-induced colorectal carcinogenesis was studied in rats maintained on a low- or a high-fat diet. Far this purpose, 120 rats received a semisynthetic diet without (Groups A and C) or with a vegetables-fruit mixture (19.5% wt/wt, Groups B and D) for 35 weeks. Diets of Group A and B contained 20 (low) energy percent (20e%) fat, whereas diets of Groups C and D contained 40e% (high) fat. Between Weeks 4 and 9 the animals were given weekly intrarectal instillations of 6 mg MNNG/kg body wt. The colorectal adenocarcinoma incidences showed a significant decrease in animals fed high- fat diets with a vegetables-fruit mixture compared with animals fed a high- fat diet alone. Furthermore, without a vegetables-fruit mixture, diets high in fat caused a significant increase in adenocarcinoma incidence compared with diets low in fat. Although not significant, the adenoma incidences tended to be lower in animals fed a vegetables-fruit mixture than in animals maintained on a diet without this mixture. The results demonstrate that a vegetables-fruit mixture has a significant inhibitory potency on the development of colorectal tumors induced by MNNG in rats fed diets high in fat.