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Idiopathic paraproteinemia. II. Transplantation of the paraprotein- producing clone from old to young C57BL/KaLwRij mice

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Author: Radl, J. · Glopper, · Schuit, H.R.E. · Zurcher, C.
Institution: Instituut voor experimentele gerontologie TNO
Source:Journal of Immunology, 2, 122, 609-613
Identifier: 228613
Keywords: Aging · Animal · Bone Marrow · Clone Cells · Fluorescent Antibody Technique · Male · Mice · Mice, Inbred C57BL · Paraproteinemias · Paraproteins · Spleen · Transplantation, Homologous


Transplantation experiments in the C57BL/KaLwRij mouse model of idiopathic paraproteinemia (IP) showed that an IP-producing clone can be further propagated in young, lethally irradiated mice and also equally as well in nonirradiated recipients by a bone marrow and/or spleen cell transfer. The latency period before the original paraprotein was detected in the sera of recipients varied in different experiments between 1 and 9 months after transplantation. With subsequent transplantations, the 'take' frequency gradually decreased. Propagation of IP for three to four generations seems to be the final limit. In comparison to age-matched control groups, no substantial influence of the transplanted IP on the survival of the recipients was observed. In contrast, transplantation of cells from mice with a B cell lymphoma or a myeloma led to continuous propagation of the malignancy, with a high 'take' frequency, progressive development of the paraproteinemia, and a shortened survival time of the recipients. These findings indicate that IP represents in its final stage in the aging C57BL mice an intrinsic cellular defect within the affected B cell clone, which is, however, different from that found in B cell malignancies. Chemicals/CAS: Paraproteins