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Functional analysis of three plasmids from Lactobacillus plantarum

Author: Kranenburg, R. van · Golic, N. · Bongers, R. · Leer, R.J. · Vos, W.M. de · Siezen, R.J. · Kleerebezem, M.
Type:article
Date:2005
Institution: TNO Kwaliteit van Leven
Source:Applied and Environmental Microbiology, 3, 71, 1223-1230
Identifier: 238387
doi: doi:10.1128/AEM.71.3.1223-1230.2005
Keywords: Biology · Biotechnology · Biomarkers · Genes · Plants (botany) · Functional analysis · Bacteria · Carnobacterium maltaromaticum · Arsenates · Arsenites · Base Sequence · Chromosome Mapping · DNA Replication · DNA, Bacterial · Drug Resistance, Bacterial · Gene Dosage · Genes, Bacterial · Lactobacillus plantarum · Molecular Sequence Data · Plasmids · Replicon · Sequence Homology, Nucleic Acid · Transfection · Bacillus subtilis · Carnobacterium maltaromaticum · Lactobacillus plantarum · Lactococcus lactis · Lactococcus plantarum

Abstract

Lactobacillus plantarum WCFS1 harbors three plasmids, pWCFS101, pWCFS102, and pWCFS103, with sizes of 1,917, 2,365, and 36,069 bp, respectively. The two smaller plasmids are of unknown function and contain replication genes that are likely to function via the rolling-circle replication mechanism. The host range of the p WCFS101 replicon includes Lactobacillus species and Lactococcus lactis, while that of the pWCFS102 replicon also includes Carnobacterium maltaromaticum and Bacillus subtilis. The larger plasmid is predicted to replicate via the theta-type mechanism. The host range of its replicon seems restricted to L. plantarum. Cloning vectors were constructed based on the replicons of all three plasmids. Plasmid pWCFS103 was demonstrated to be a conjugative plasmid, as it could be transferred to L. plantarum NC8. It confers arsenate and arsenite resistance, which can be used as selective markers. Copyright © 2005, American Society for Microbiology. All Rights Reserved.