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Insertion of pea lectin into a phospholipid monolayer

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Author: Booij, P. · Demel, R.A. · Pater, B.S. de · Kijne, J.W.
Type:article
Date:1996
Institution: Centraal Instituut voor Voedingsonderzoek TNO TNO Voeding
Source:Plant Molecular Biology, 1, 31, 169-173
Identifier: 280544
Keywords: Nutrition · Monolayer · Pea lectin · Phosphatidylcholine · Wilhelmy plate method · Pea lectin · Phospholipid · Plant lectin · Metabolism · PH · Protein binding · Carbohydrate Metabolism · Cell Membrane · Hydrogen-Ion Concentration · Lectins · Phospholipids · Plant Lectins · Plant Roots · Protein Binding · Pisum sativum

Abstract

Pea lectin (PSL) is a secretory sugar-binding protein, readily soluble in aqueous solutions of low osmolarity. However, PSL also appears to be associated with the plasma membrane at the tip of young pea root hairs. By using the Wilhelmy plate method, we found that PSL can insert into a lipid monolayer. This property appeared to be independent of the sugar- binding ability of the protein. This result suggests that PSL may be directly involved in membrane-mediated interactions with saccharide ligands, for example during root hair infection by symbiotic rhizobia. Chemicals/CAS: Lectins; pea lectin; Phospholipids; Plant Lectins