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Unlocking Streptomyces spp. for use as sustainable industrial production platforms by morphological engineering

Author: Wezel, G.P. van · Krabben, P. · Traag, B.A. · Keijser, B.J.F. · Kerste, R. · Vijgenboom, E. · Heijnen, J.J. · Kraal, B.
Institution: TNO Kwaliteit van Leven
Source:Applied and Environmental Microbiology, 8, 72, 5283-5288
Identifier: 239412
doi: doi:10.1128/AEM.00808-06
Keywords: Biology · Biotechnology · Antibiotics · Batch cell culture · Enzymes · Fermentation · Growth kinetics · Microbiology · Morphology · Industrial enzymes · Morphological engineering · Natural products · Pellet formation · Bacteria · antibiotic agent · industrial enzyme · natural product · antibiotics · bacterium · enzyme · fermentation · gene expression · growth rate · morphology · Actinobacteria · article · batch fermentation · bioengineering · controlled study · culture medium · environmental sustainability · enzyme synthesis · fungal gene · fungus growth · gene expression · morphological engineering · mycelium · nonhuman · ssgA gene · Streptomyces · Streptomyces coelicolor · Streptomyces lividans · Bacterial Proteins · Culture Media · Fermentation · Gene Expression Regulation, Bacterial · Genetic Engineering · Industrial Microbiology · Streptomyces · Actinobacteria (class) · Streptomyces · Streptomyces coelicolor · Streptomyces lividans · Streptomycineae


Filamentous actinomycetes are commercially widely used as producers of natural products (in particular antibiotics) and of industrial enzymes. However, the mycelial lifestyle of actinomycetes, resulting in highly viscous broths and unfavorable pellet formation, has been a major bottleneck in their commercialization. Here we describe the successful morphological engineering of industrially important streptomycetes through controlled expression of the morphogene ssgA. This led to improved growth of many industrial and reference streptomycetes, with fragmentation of the mycelial clumps resulting in significantly enhanced growth rates in batch fermentations of Streptomyces coelicolor and Streptomyces lividans. Product formation was also stimulated, with a twofold increase in yield of enzyme production by S. lividans. We anticipate that the use of the presented methodology will make actinomycetes significantly more attractive as industrial and sustainable production hosts. Copyright © 2006, American Society for Microbiology. All Rights Reserved. Chemicals / CAS: Bacterial Proteins; Culture Media; ssgA protein, bacterial