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Infection of spheroplasts of Pseudomonas with DNA of bacteriophage PM2

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Author: Schans, G.P. van der · Weyermans, J.P. · Bleichrodt, J.F.
Institution: Medisch Biologisch Laboratorium TNO
Source:MGG Molecular & General Genetics, 3, 110, 263-271
Identifier: 227280
doi: doi:10.1007/BF00337838
Keywords: Biology · Ion · Lysozyme · Virus DNA · Bacteriophage · Bacteriophage typing · Growth, development and aging · Metabolism · Microbial genetics · Pseudomonas · Bacteriophage Typing · Bacteriophages · DNA, Viral · Genetics, Microbial · Ions · Muramidase · Pseudomonas


Spheroplasts of Pseudomonas BAL-31/PM2, obtained by treatment of the bacteria with lysozyme, can be infected with purified DNA from bacteriophage PM2. After 4 h of incubation the yield of progeny phage reaches a value of 107-6×107 plaque forming units/μg PM2 DNA. The yield increases linearly with the concentration of DNA over at least 3 orders of magnitude. The biological activity of double-stranded circular PM2 DNA containing one or more single-strand breaks per molecule (component II), does not differ significantly from that of intact PM2 DNA (component I). Single-stranded PM2 DNA obtained by denaturation of component II, and the irreversible alkali-denatured form of component I are also infective. © 1971 Springer-Verlag.