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Usefulness and limitations of Actilyse® for standardisation of tissue-type plasminogen activator (t-PA) assays in blood

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Author: Huisman, L.G.M. · Griensven, J.M.T. van · Kluft, C.
Type:article
Date:1996
Institution: TNO Preventie en Gezondheid
Source:Fibrinolysis, SUPPL. 2, 10, 125-127
Identifier: 233525
Keywords: Biology · Alteplase · Tissue plasminogen activator · Assay · Blood level · Conference paper · Controlled study · Fibrinolysis · Human · Standardization

Abstract

Standardisation of t-PA antigen and activity assays requires a t-PA standard which ideally is a single preparation suitable for all assays. Most t-PA preparations, however, are not homogeneous; they can contain several forms of t-PA: single-chain and two-chain t-PA, and two glycoforms (I and II). In this study, the behaviour of Actilyse recombinant t-PA in several assays was studied and especially the effect of the two glycosylation forms on the efficiency of the assay. In two antigen assays and a direct assay on a chromogenic substrate we observed no specificity difference for different molecular forms of Actilyse whereas, in two-step assays based on plasminogen activation, such differences were present. It is concluded that for each assay the sensitivity to different forms of t-PA should be determined, in order to be able to decide whether or not Actilyse is a suitable standard preparation.