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mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

Author: Slagboom, P.E. · Leeuw, · Vijg, J.
Institution: Instituut voor Experimentele Gerontologie TNO
Source:FEBS Letters, 1, 269, 128-130
Identifier: 231214
doi: doi:10.1016/0014-5793(90)81136-C
Article number: 08746
Keywords: DNA methylation · mRNA · Tyrosine aminotransferase · Aging · Animal · Blotting, Northern · Blotting, Southern · Gene Expression · Genes, Structural · Liver · Methylation · Rats · RNA, Messenger · Tyrosine Transaminase · Animalia


We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.