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The production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi

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Author: Joosten, V. · Lokman, C. · Hondel, C.A.M.J.J. van den · Punt, P.J.
Type:article
Date:2003
Institution: TNO Voeding
Source:Microbial Cell Factories, 2
Identifier: 236944
doi: doi:10.1186/1475-2859-2-1
Article number: No.: 1
Keywords: Biology · Biotechnology · cancer antibody · cell antibody · fungus antibody · hybrid protein · immunoglobulin · immunoglobulin F(ab) fragment · immunoglobulin Fc fragment · immunoglobulin heavy chain · immunoglobulin light chain · intrabody · recombinant antibody · single chain fragment variable antibody · unclassified drug · antibody combining site · antibody production · antigen binding · Aspergillus awamori · Candida boidinii · clinical trial · drug clearance · drug delivery system · Escherichia coli · eukaryote · gene expression system · Hansenula polymorpha · human · immunotherapy · Kluyveromyces · methylotrophic bacterium · mycology · nonhuman · Pichia · Pichia methanolica · Pichia pastoris · protein protein interaction · protein synthesis · review · Saccharomyces cerevisiae · Schizosaccharomyces pombe · Trichoderma reesei · Yarrowia lipolytica · Eukaryota · Fungi

Abstract

In this review we will focus on the current status and views concerning the production of antibody fragments and antibody fusion proteins by yeasts and filamentous fungi. We will focus on single-chain antibody fragment production (scFv and VHH) by these lower eukaryotes and the possible applications of these proteins. Also the coupling of fragments to relevant enzymes or other components will be discussed. As an example of the fusion protein strategy, the 'magic bullet' approach for industrial applications, will be highlighted. © 2003 Joosten et al; licensee BioMed Central Ltd.