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Apolipoprotein A-IV polymorhism and its effect on plasma lipid and apolipoprotein concentrations

Author: Knijff, P. de · Rosseneu, M. · Beisiegel, U. · Keersgieter, W. de · Frants, R.R. · Havekes, L.M.
Institution: Gaubius Instituut TNO
Source:Journal of Lipid Research, 12, 29, 1621-1627
Identifier: 230747
Keywords: Biology · Triacylglycerol · Apolipoprotein blood level · Heredity · Lipid blood level · Population genetics · Protein polymorphism · Blood · Genetic polymorphism · Genetics · Phenotype · Adult · Alleles · Animal · Apolipoproteins · Apolipoproteins A · Apolipoproteins B · Cholesterol · Gene Frequency · Human · Lipids · Male · Phenotype · Polymorphism (Genetics) · Rabbits · Triglycerides


Recently, we determined the apolipoprotein E (apoE) phenotype distribution in 2,000 randomly selected 35-year-old male individuals by slab gel isoelectric focusing of delipidated plasma samples, followed by immunoblotting using anti-apoE antiserum. These blots have been successfully re-used for immunovisualization of apoA-IV isoelectric focusing patterns. In a population sample of 1,393 individuals, four distinct apoA-IV isoforms were detected, encoded by the alleles A-IV*0, A-IV*1, A-IV*2, and A-IV*3 with gene frequencies of 0.002, 0.901, 0.079, and 0.018, respectively. The mean of plasma cholesterol, triglyceride, apoB and E levels did not differ significantly among the different apoA-IV phenotype groups. For these lipoprotein parameters, less than 0.1% of the total phenotypic variance could be accounted for by the APOA-IV gene locus. Our results did not show any effect of apoA-IV polymorphism on plasma apoA-I levels nor could we find any correlation between plasma levels of apoA-I and apoA-IV within the different apoA-IV phenotype groups. The plasma level of apoA-IV in subjects bearing the A-IV*3 allele is significantly lower than in subjects without the A-IV*3 allele (5 mg/dl versus 14 mg/dl). We therefore conclude that, in contrast to the apoE polymorpism, the polymorphism at the APOA-IV locus does not influence any of the level of the lipoprotein parameters considered except apoA-IV. Chemicals/CAS: cholesterol, 57-88-5; lipid, 66455-18-3; apolipoprotein A-IV; Apolipoproteins A; Apolipoproteins B; Apolipoproteins; Cholesterol, 57-88-5; Lipids; Triglycerides