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Maintenance of bile acid synthesis and cholesterol 7α-hydroxylase activity in cultured rat hepatocytes

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Author: Princen, H.M.G. · Meijer, P.
Type:article
Date:1990
Institution: Gaubius instituut TNO
Source:Biochemical Journal, 1, 272, 273-275
Identifier: 231366
doi: doi:10.1042/bj2720273
Keywords: Health · Animal · Bile Acids and Salts · Cells, Cultured · Cholesterol 7-alpha-Hydroxylase · Culture Media · Liver · Male · Rats · Rats, Inbred Strains · Animalia · Bovinae

Abstract

Addition of foetal-bovine serum to rat hepatocytes cultured in Williams E medium resulted in improved maintenance of bile-acid-synthetic capacity and cholesterol 7α-hydroxylase activity as compared with cultures supplemented with rat or newborn-bovine serum or cultures in a hormonally defined serum-free medium. Minimally, 5% (v/v) foetal-bovine serum was necessary to maintain these liver-specific functions. Serum factor(s) responsible for these effects were not dialysable or associated with lipoproteins, but were removed by charcoal extraction. Chemicals/CAS: Bile Acids and Salts; Cholesterol 7-alpha-Hydroxylase, EC 1.14.13.17; Culture Media