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Effects of all-trans retinol and cigarette smoke condensate on hamster tracheal epithelium in organ culture. II. A histomorphological study

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Author: Rutten, A.A.J.J.L. · Beems, R.B. · Wilmer, J.W.G.M.
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Virchows Archiv Abteilung B Cell Pathology, 3, 55, 177-186
Identifier: 230563
Keywords: cigarette smoke condensate · dimethyl sulfoxide · retinol · animal cell · animal experiment · histology · nonhuman · organ culture · squamous cell metaplasia · trachea mucosa · ultrastructure · Animal · Cell Differentiation · Dimethyl Sulfoxide · Drug Interactions · Epithelium · Hamsters · Hyperplasia · Mesocricetus · Metaplasia · Organ Culture · Plants, Toxic · Smoke · Support, Non-U.S. Gov't · Tobacco · Trachea · Vitamin A · Vitamin A Deficiency


The effects of all-trans retinol and cigarette smoke condensate (CSC) on tissue morphology and cellular differentiation were investigated in vitamin A-deprived tracheal epithelium cultured in vitamin A- and serum-free hormone-supplemented medium. Physiological retinol concentrations prevented the development of hyperplasia and squamous metaplasia with or without keratinization, and induced differentiation to mucous cells. Squamous metaplastic foci with keratinization were observed during 12 days of culture with low retinol concentrations and with dimethylsulfoxide (DMSO) which was accompanied by an increased number of basal and indeterminate cells. CSC induced a dose-related hyperplasia and irregularly shaped foci of squamous metaplasia with atypical epithelial proliferation. In non-metaplastic epithelium, CSC exposure increased the number of ciliated cells. Hyperplasia and squamous metaplasia were inhibited if the tracheal rings were first treated with retinol followed by CSC exposure, or if the tracheas were simultaneously treated with retinol and CSC. CSC-exposure prior to retinol treatment induced similar histomorphological alterations as CSC alone. Chemicals/CAS: dimethyl sulfoxide, 67-68-5; retinol, 68-26-8, 82445-97-4; Dimethyl Sulfoxide, 67-68-5; Vitamin A, 11103-57-4