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Arginine-dependent acid resistance in Salmonella enterica serovar typhimurium

Author: Kieboom, J. · Abee, T.
Type:article
Date:2006
Institution: TNO Defensie en Veiligheid
Source:Journal of Bacteriology, 15, 188, 5650-5653
Identifier: 239406
doi: doi:10.1128/JB.00323-06
Keywords: Antiporter · Acidity · AdiA gene · AdiC gene · AdiY gene · Anaerobic growth · Anoxia · Bacterial gene · Bacterium mutant · Enzyme activity · Escherichia coli · Genetic transcription · Nonhuman · Salmonella enterica · Adaptation, Physiological · Hydrogen-Ion Concentration · Mutation · Salmonella typhimurium · Adia · Escherichia coli · Salmonella · Salmonella enterica · Salmonella enterica subsp. enterica serovar Typhimurium · Agmatine, 306-60-5 · Arginine decarboxylase, 9024-77-5 · Arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3 · Arginine decarboxylase, EC 4.1.1.19 · Carboxy-Lyases, EC 4.1.1.- · Oxygen, 7782-44-7

Abstract

Salmonella enterica serovar Typhimurium does not survive a pH 2.5 acid challenge under conditions similar to those used for Escherichia coli (J. W. Foster, Nat. Rev. Microbiol. 2:898-907, 2004). Here, we provide evidence that S. enterica serovar Typhimurium can display arginine-dependent acid resistance (AM) provided the cells are grown under anoxic conditions and not under the microaerobic conditions used for assessment of AR in E. coli. The role of the arginine decarboxylase pathway in Salmonella AR was shown by the loss of AR in mutants lacking adiA, which encodes arginine decarboxylase; adiC, which encodes the arginine-agmatine antiporter; or adiY, which encodes an AraC-like regulator. Transcription of adiA and adiC was found to be dependent on AdiY, anaerobiosis, and acidic pH. Copyright © 2006, American Society for Microbiology. All Rights Reserved.