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Hexachlorobenzene and its metabolites pentachlorophenol and tetrachlorohydroquinone: Interacion with thyroxine binding sites of rat thyroid hormone carriers ex vivo and in vitro

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Author: Raaij, J.A.G.M. van · Berg, K.J. van den · Notten, W.R.F.
Type:article
Date:1991
Institution: Medisch Biologisch Laboratorium TNO
Source:Toxicology Letters, 1-3, 59, 101-107
Identifier: 231701
doi: doi:10.1016/0378-4274(91)90060-J
Keywords: Biology · Binding sites · Carrier · Hexachlorobenzene · Occupation · Pentachlorophenol · Tetrachlorohydroquinone · Th yroxine · Hexachlorobenzene · Pentachlorophenol · Tetrachlorohydroquinone · Thyroxine · Thyroxine binding protein · Animal experiment · Animal tissue · Hypothyroidism · Metabolite · Nonhuman · Priority journal · Rat · Animal · Binding Sites · Binding, Competitive · Hexachlorobenzene · Hydroquinones · Hypothyroidism · Injections, Intraperitoneal · Pentachlorophenol · Rats · Support, Non-U.S. Gov't · Thyroxine

Abstract

Previous results have indicated that hexachlorobenzene (HCB)-induced hypothyroidism may be caused by its main metabolite pentachlorophenol (PCP), and by tetrachlorohydroquinone (TCHQ), rather than by the parent compound. In the present experiments it was investigated whether hormone displacement from serum carriers could be a factor in the development of this hypothyroidism. In an in vitro competition assay PCP was an effective competitor for the thyroxine (T4)-binding sites of serum carriers, whereas HCB was ineffective. Ex vivo experimental results demonstrated occupation of T4-binding sites in sera from PCP-exposed animals but not in sera from HCB-or TCHQ-treated animals. Competing ability for T4-binding sites was still present in sera of PCP-exposed animals but was absent in HCB- or TCHQ-exposed animals. The results suggest that thyroid hormone displacement by the major metabolite PCP may play a role in HCB-induced hypothyroidism. Chemicals/CAS: 2,3,5,6-tetrachlorohydroquinone, 87-87-6; Hexachlorobenzene, 118-74-1; Hydroquinones; Pentachlorophenol, 87-86-5; Thyroxine, 7488-70-2