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Biochemistry and measurement of fibrinogen

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Author: Nieuwenhuizen, W.
Type:article
Date:1995
Institution: TNO Preventie en Gezondheid
Source:European Heart Journal, SUPPL. A, 16, 6-10
Identifier: 232844
Keywords: Biology · Fibrinogen · Functionality of fibrinogen · Measurement · Molecular heterogeneity · Polymerization · disulfide · fibrinogen · acute heart infarction · blood clotting · disulfide bond · enzyme immunoassay · fibrinolytic therapy · ischemic heart disease · molecular weight · priority journal · protein polymorphism · review · risk · thrombosis · Cardiovascular Diseases · Coronary Disease · Fibrinogen · Human · Immunoassay · Molecular Weight · Precipitation · Reference Values · Whole Blood Coagulation Time

Abstract

Fibrinogen is a large heterogeneous family of closely related molecules consisting of three pairs of non-identical polypeptide chains: two Aα-, two Bβ- and two γ-chains, held together by disulphide bridges. The heterogeneity of fibrinogen is due to heterogeneities in all three chains. Four main types of assay are used to determine fibrinogen:clotting rate (Clauss), clottable protein, precipitation and immunological assays. Heterogenities may differ from person to person and may affect the apparent fibrinogen concentrations in different assays. A further complicating factor was, until recently, the lack of an international fibrinogen standard. The ratio of Clauss:enzyme immunoassay (EIA) for high + low molecular weight fibrinogen decreases during therapy for acute myocardial infarction and increases again after thrombolytic therapy to above normal values. Furthermore, high molecular weight fibrinogen tends to clot more easily than low molecular weight fibrinogen. This suggests that high molecular weight fibrinogen might be associated with increased thrombolytic risk. Fibrinogen assessed by a functional assay (Clauss) alone is strongly associated with ischaemic heart disease. Although not proven, it is conceivable that a fibrinogen with a Clauss:EIA ratio of > 1 has an even stronger association in epidemiological studies. Chemicals/CAS: disulfide, 16734-12-6; fibrinogen, 9001-32-5; Fibrinogen, 9001-32-5