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Peanut allergen Ara h 1 interacts with proanthocyanidins into higher molecular weight complexes

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Author: Boxtel, E.L. van · Broek, L.A.M. van den · Koppelman, S.J. · Vincken, J.-P. · Gruppen, H.
Type:article
Date:2007
Institution: TNO Kwaliteit van Leven
Source:Journal of Agricultural and Food Chemistry, 21, 55, 8772-8778
Identifier: 240250
doi: doi:10.1021/jf071585k
Keywords: Food technology · Ara h 1 · Peanut allergy · Proanthocyanidins · Protein-polyphenol interaction · allergen · Ara h 1 protein, Arachis hypogaea · beta conglycinin protein, Glycine max · beta-conglycinin protein, Glycine max · globulin · glycinin · glycoprotein · proanthocyanidin · soybean protein · vegetable protein · article · chemical structure · chemistry · drug interaction · gel chromatography · ion exchange chromatography · molecular weight · Allergens · Chromatography, Gel · Chromatography, Ion Exchange · Drug Interactions · Globulins · Glycoproteins · Models, Molecular · Molecular Weight · Plant Proteins · Proanthocyanidins · Soybean Proteins · Ara · Arachis hypogaea · Glycine max

Abstract

Mildly extracted peanut allergen Ara h 1 was previously reported to occur as an oligomeric complex. In this paper we describe how the protein in this oligomeric complex interacts noncovalently with phenolic compounds of the proanthocyanidin type. These interactions are being disrupted during anion exchange chromatography, resulting in the dissociation of the oligomeric Ara h 1 complex into protein trimers. By use of the known three-dimensional structure of β-conglycinin, a soy protein homologous to Ara h 1, proline-rich regions were observed in silico on both faces of its trimeric structure, which are conserved in Ara h 1. These proline-rich regions could explain the binding of proanthocyanidins to Ara h 1 and the formation of multiple Ara h 1 trimer complexes. This was supported by the observation that the addition of peanut proanthocyanidins to trimeric Ara h 1 and to β-conglycinin resulted in the formation of soluble oligomeric protein complexes. The structurally related legumin proteins do not contain such proline-rich regions on both sides of the protein, and proanthocyanidins were shown to have a lower affinity for legumin proteins from peanuts and soybeans (peanut allergen Ara h 3 and soy glycinin, respectively). Ara h 1 present as the oligomeric complex is assumed to be the representative form of the allergen in which it is consumed by humans. © 2007 American Chemical Society.