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Experimental hypothyroidism increases plasminogen activator inhibitor activity in rat plasma

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Author: Padró, T. · Hoogen, C.M. van den · Emeis, J.J.
Institution: Instituut voor verouderings- en vaatziekten onderzoek TNO
Source:Blood Coagulation and Fibrinolysis, 5, 4, 797-800
Identifier: 232119
Keywords: Biology · fibrinolysis · thyroid hormone replacement · cholesterol · levothyroxine · urokinase · animal experiment · animal model · blood level · controlled study · hypothyroidism · nonhuman · oral drug administration · Animal · Cholesterol · Comparative Study · Hypothyroidism · Male · Plasminogen Inactivators · Rats · Rats, Wistar · Thiouracil · Thyroxine · Tissue Plasminogen Activator · Triiodothyronine · Urinary Plasminogen Activator


The aim of the present study was to investigate whether hypothyroidism would affect the components of the rat plasma fibrinolytic system. Hypothyroidism was induced by feeding rats a thiouracil-containing diet for 2 weeks. During this period, half of the animals received subcutaneous injections of L-thyroxine (20 μg/kg daily) to restore thyroid hormone levels. Groups of euthyroid rats were similarly treated with L-thyroxine. Plasma tissue-type PA (t-PA) activity was not affected by thyroid status. Plasma urokinase-type PA (u-PA) activity was significantly decreased by the thiouracil-containing diet; however, this effect was not abolished by L-thyroxine. Plasminogen, α2-antiplasmin and fibrinogen were not affected by hypothyroidism. In the hypothyroid group, plasma PAI activity was significantly increased; no such increase was found in animals simultaneously receiving L-thyroxine. Plasma PAI activity correlated with T3 (r = -0.586; P < 0.005) and cholesterol (r = +0.737; P < 0.001); in multiple regression analysis, however, the correlation between PAI activity and T3 was not significant. The increase in plasma PAI activity during hypothyroidism may thus not be a direct effect of the thyroid hormone, but an indirect one through some other, possibly cholesterol related, component. Chemicals/CAS: cholesterol, 57-88-5; levothyroxine, 51-48-9; plasminogen activator inhibitor, 105844-41-5; thiouracil, 141-90-2; tissue plasminogen activator, 105913-11-9; urokinase, 139639-24-0; Cholesterol, 57-88-5; Plasminogen Inactivators; Thiouracil, 141-90-2; Thyroxine, 7488-70-2; Tissue Plasminogen Activator, EC; Triiodothyronine, 6893-02-3; Urinary Plasminogen Activator, EC