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Functional expression in Lactobacillus plantarum of xylP encoding the isoprimeverose transporter of Lactobacillus pentosus

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Author: Chaillou, S. · Postma, P.W. · Pouwels, P.H.
Type:article
Date:1998
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Journal of Bacteriology, 15, 180, 4011-4014
Identifier: 234582
Keywords: Nutrition · Carbohydrate · Carrier protein · Isoprimeverose · Unclassified drug · Xylose · Carbohydrate transport · Controlled study · Fermentation · Gene expression · Lactobacillus · Lactobacillus pentosus · Lactobacillus plantarum · Nonhuman · Priority journal · Proton motive force · Bacterial Proteins · Biological Transport · Carrier Proteins · Cloning, Molecular · Disaccharides · Kinetics · Lactobacillus · Malates · Operon · Plasmids · Recombinant Proteins · Restriction Mapping · Symporters · Xylose

Abstract

The xylP gene of Lactobacillus pentosus, the first gene of the xylPQR operon, was recently found to be involved in isoprimeverose metabolism. By expression of xylP on a multicopy plasmid in Lactobacillus plantarum 80, a strain which lacks active isoprimeverose and D-xylose transport activities, it was shown that xylP encodes a transporter. Functional expression of the XylP transporter was shown by uptake of isoprimeverose in L. plantarum 80 cells, and this transport was driven by the proton motive force generated by malolactic fermentation. XylP was unable to catalyze transport of D-xylose.