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Abscisic acid induces a cytosolic calcium decrease in barley aleurone protoplasts

Author: Wang, M. · Duijn, B. van · Schram, A.W.
Type:article
Date:1991
Institution: Instituut voor Biotechnologie en Chemie TNO
Source:FEBS Letters, 1, 278, 69-74
Identifier: 231480
doi: doi:10.1016/0014-5793(91)80086-I
Keywords: Barley aleurone · Dormancy · Gibberellic acid · Cadmium · Lanthanide · Plant · Abscisic Acid · Ca(2+)-Transporting ATPase · Calcium · Cell Membrane · Cytosol · Enzyme Activation · Fluorescent Dyes · Hordeum · Indoles · Nifedipine · Protoplasts · Support, Non-U.S. Gov't · Verapamil · Hordeum vulgare subsp. vulgare

Abstract

Cytosolic calcium concentrations (Ca(i)) of barley aleurone protoplasts after stimulation with the plant hormone abscisic acid (ABA) were measured by using the calcium-sensitive fluorescent dye Indo-1. The measuredd basal Ca(i) is about 200 nM. Stimulation with ABA induces a strong dose-dependent decrease in Ca(i) to a minimal value of about 50 nM. This decrease occurs within 5 s. The Ca2+ antagonists La3+ and Cd2+ inhibit the ABA-induced Ca(i) decrease in a dose-dependent manner, while the Ca2+ channel blockers verapamil and nifedipine give no inhibition. The induction of Ca(i) decrease by ABA is consistent with activation of the plasma membrane Ca2+-ATPase by ABA. The possible role of this ABA-induced Ca(i) decrease in ABA signal transduction and in counteracting the effects of gibberellic acid are discussed. Chemicals/CAS: Histocompatibility Antigens Class IIChemicals/CAS: Abscisic Acid, 21293-29-8; Ca(2+)-Transporting ATPase, EC 3.6.1.38; Calcium, 7440-70-2; Fluorescent Dyes; indo-1, 96314-96-4; Indoles; Nifedipine, 21829-25-4; Verapamil, 52-53-9