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The promoter of the rice gene GOS2 is active in various different monocot tissues and binds rice nuclear factor ASF-1

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Author: Pater, Sylvia B. de · Mark, F. van der · Rueb, S. · Katagiri, F. · Chua, N.H. · Schilperoort, R.A. · Hensgens, L.A.M.
Type:article
Date:1992
Institution: Centrum voor Plantenfysiologisch Onderzoek
Source:Plant Journal, 6, 2, 837-844
Identifier: 231994
Keywords: Beta glucuronidase · GOS2 protein, Oryza sativa · Splicing factor 2 · Vegetable protein · Biosynthesis · Comparative study · Genetics · Metabolism · Molecular cloning · Molecular genetics · Promoter region · Rice · Amino Acid Sequence · Base Sequence · Cloning, Molecular · Comparative Study · Genes, Plant · Genetic Engineering · Glucuronidase · Molecular Sequence Data · Nuclear Proteins · Oryza sativa · Plant Proteins · Plants · Promoter Regions (Genetics) · Recombinant Proteins · Restriction Mapping · Sequence Homology, Amino Acid · Support, Non-U.S. Gov't · Tissue Distribution

Abstract

A single copy gene has been isolated, termed GOS2, from rice. Sequence comparison revealed highly similar genes in mammals and yeast, indicating that GOS2 encodes an evolutionary conserved protein. GOS2 mRNA was detected in all tissues examined. When the upstream region was translationally fused to the reporter gene gusA it was found to drive expression in a variety of rice tissues and in cell suspensions of other monocot species following introduction by particle bombardment. Therefore, the GOS2 promoter is potentially useful for genetic engineering of monocots. A DNA-binding activity from rice, termed rice ASF-1, with similar binding specificity as the cloned tobacco transcription factor TGA-1a, was found to bind to a TGACG sequence motif in the GOS2 promoter. Possible roles for rice ASF-1 in the transcriptional activation of the GOS2 promoter are discussed. Chemicals/CAS: beta glucuronidase, 9001-45-0; Glucuronidase, EC 3.2.1.31; GOS2 protein, Oryza sativa; Nuclear Proteins; Plant Proteins; Recombinant Proteins; splicing factor 2