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Detection of zilpaterol (Zilmax®) in calf urine and faeces with liquid chromatography-tandem mass spectrometry

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Author: Hoof, N. van · Schilt, R. · Vlis, E. van der · Boshuis, P. · Baak, M. van · Draaijer, A. · Wasch, K. de · Wiele, M. van de · Hende, J.V. van · Courtheyn, D. · Brabander, H. de
Institution: TNO Kwaliteit van Leven
Source:Analytica Chimica Acta, 1-2 SPEC. ISS., 529, 189-197
Identifier: 238312
doi: doi:10.1016/j.aca.2004.08.072
Keywords: Toxicology · Analytical research · Beta-agonists · Excretion profile · LC-MSn · Zilpaterol · Body fluids · Liquid chromatography · Mass spectrometry · Optimization · Calf urine · Faeces · Fattening · Zilpaterol · Alcohols · beta adrenergic receptor stimulating agent · unclassified drug · zilmax · zilpaterol · animal experiment · animal tissue · cattle · conference paper · controlled study · drug determination · drug excretion · drug structure · gas chromatography · liquid chromatography · male · mass spectrometry · nonhuman · priority journal · tandem mass spectrometry · urinalysis · Bos taurus


Zilpaterol is a new powerful beta-agonist, which is officially registered for fattening purposes in cattle in Mexico and South Africa. Its chemical structure is different from the well-known beta-agonists. Therefore, the routinely used screening methods are not likely to be suited for the analysis of zilpaterol. Also gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry (LC-MS) methods need to be adapted to enable detection of zilpaterol. In this study, a LC-MS3 confirmatory method was developed for the simultaneous detection of zilpaterol and di-aromatic beta-agonists in urine samples. A LC-MS2 method was optimised for the detection of zilpaterol in faeces. To study the excretion profile in urine and faeces, a male veal calf was orally treated with daily doses of Zilmax ® during 2 weeks. Zilpaterol was mainly excreted via urine. © 2004 Elsevier B.V. All rights reserved.