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Factors determining cell killing by chemotherapeutic agents In Vivo-II : Melphalan, chlorambucil and nitrogen mustard

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Author: Putten, L.M. van · Lelieveld, P.
Institution: Radiobiologisch Instituut TNO
Source:European Journal of Cancer, 1, 7, 11-16
Identifier: 227235
Keywords: Drug effect · female · hematopoietic stem cell · metabolism · Animal · Bone Marrow · Bone Marrow Cells · Cell Survival · Chlorambucil · Cyclophosphamide · DNA · Female · Hematopoietic Stem Cells · Male · Mechlorethamine · Melphalan · Mice · Spleen


The sensitivity of resting spleen colony-forming cells in normal mouse bone marrow and of rapidly-proliferating spleen colony-forming cells in repopulating mouse spleen to a number of bis-chloro-aethyl-amines was determined. The rapidly-growing cells were more sensitive to each of the agents than the resting cells. The equal degree of sensitization to these agents by an increased rate of proliferation of the cells points to a common mechanism for all agents of this type. It is suggested that repair of damaged DNA, by excision of alkylated bases or oligonucleotides before the damage becomes irreversible during the next DNA synthetic period, is the most likely explanation of the uniform difference in sensitivity caused by the modified rate of cell proliferation. © 1971. Chemicals/CAS: chlorambucil, 305-03-3; chlormethine, 51-75-2, 55-86-7, 82905-71-3; cyclophosphamide, 50-18-0; DNA, 9007-49-2; melphalan, 148-82-3; Chlorambucil, 305-03-3; Cyclophosphamide, 50-18-0; DNA, 9007-49-2; Mechlorethamine, 51-75-2; Melphalan, 148-82-3