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Automated electron tomography of the septal pore cap in Rhizoctonia solani

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Author: Müller, W.H. · Koster, A.J. · Humbel, B.M. · Ziese, U. · Verkleij, A.J. · Aelst, A.C. van · Krift, T.P. van der · Montijn, R.C. · Boekhout, T.
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Journal of Structural Biology, 1, 131, 10-18
Identifier: 72003
doi: doi:10.1006/jsbi.2000.4243
Keywords: Nutrition · Botany · Fungi · β-1,6-glucan · Cryo-scanning electron microscopy · Dolipore septum · Electron tomography · Filamentous structures · Freeze substitution · Fungus · High-pressure freezing · Rhizoctonia solani · Septal pore cap · Transmission electron microscopy · ZIO staining · Automation · Cell communication · Cell ultrastructure · Channel gating · Diagnostic value · Electron beam tomography · Hyphomycetes · Membrane structure · Nonhuman · Priority journal · Automation · Cell Membrane · Cryoelectron Microscopy · Electrons · Endoplasmic Reticulum · Freezing · Image Processing, Computer-Assisted · Intercellular Junctions · Intracellular Membranes · Microscopy, Electron · Microscopy, Electron, Scanning · Mitochondria · Rhizoctonia · Tomography · Basidiomycota · Fungi · Hyphomycetes · Rhizoctonia · Thanatephorus cucumeris


Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hypal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the SPC with associated cell ultrastructures in three dimensions by automated electron tomography of thick-sectioned cells, followed by 3D tomographic reconstructions. Using these methods we were able to document the passage of mitochondria through the SPC, small tubular membranous structures at the entrance of the septal pore channel, filamentous structures connecting the inner side of the SPC with pore-plugging material, thin filaments anchoring the pore-plugging material with the plasma membrane, small vesicles attached to the plugging material, and tubular endoplasmic reticulum continuous with the base of the SPC. We hypothesize that the SPC, the filamentous structures, the plugging material, and the endoplasmic reticulum act in a coordinated fashion to maintain cellular integrity, intercellular communication, and the transport of solutes and cell organelles in the filamentous fungus R. solani. (C) 2000 Academic Press.