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Biological monitoring the exposure to polycyclic aromatic hydrocarbons of coke oven workers in relation to smoking and genetic polymorphisms for GSTM1 GSTT1

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Author: Delft, J.H.M. van · Steenwinkel, M.-J.S.T. · Asten, J.G. van · Vogel, N. de · Bruijntjes-Rozier, T.C.D.M. · Schouten, T. · Cramers, P. · Maas, L. · Herwijnen, M.H. van · Schooten, F.-J. van · Hopmans, P.M.J.
Type:article
Date:2001
Institution: Centraal Instituut voor Voedingsonderzoek TNO
Source:Annals of Occupational Hygiene, 5, 45, 395-408
Identifier: 41992
Keywords: Biology · 1-OHpyr: 1-hydroxypyrene · GST: glutathione S-transferase · HFC: cells with a high frequency of SCE · MN: micronuclei · PAH: polycyclic aromatic hydrocarbons · SCE: sister-chromatid exchange · 1 Hydroxypyrene · Glutathione transferase · Polycyclic aromatic hydrocarbon · Adult · Biological monitoring · Cancer risk · Chromosome analysis · Coke oven industry · Comet assay · Controlled study · DNA adduct · DNA strand breakage · Endothelium cell · Genetic polymorphism · Human · Industrial worker · Industry · Lung cancer · Lymphocyte · Male · Micronucleus test · Multivariate analysis · Normal human · Occupational exposure · Priority journal · Sister chromatid exchange · Smoking · Urine level · Adult · Coke · DNA Adducts · Glutathione Transferase · Humans · Middle Aged · Multivariate Analysis · Occupational Exposure · Polycyclic Hydrocarbons, Aromatic · Polymorphism, Genetic · Sister Chromatid Exchange · Smoking

Abstract

Occupational exposure to polycyclic aromatic hydrocarbons (PAH) increases the risk of developing lung cancer. Human exposure is often demonstrated by increased internal levels of PAH metabolites and of markers for early biological effects, like DNA adducts and cytogenetic aberrations. Objective: This study aimed to assess whether the current exposure to PAH of coke oven workers in a Dutch plant induced biological effects, and to determine if these effects are influenced by tobacco smoking and by genetic polymorphisms for the glutathione S-transferase genes GSTM1 and GSTT1. Methods: Urinary 1-hydroxypyrene (1-OHpyr) levels were used to monitor the internal dose, while the internal effective dose was assessed by monitoring PAH-DNA adducts, DNA strand breaks (Comet assay), sister-chromatid exchanges (SCE) and cells with a high frequency of SCE (HFC) in lymphocytes together with micronuclei (MN) in exfoliated urothelial cells. Results: Occupational exposure to PAH resulted in statistically significant increased 1-OHpyr levels (P<0.001), but it did not cause a significant induction of SCE, HFC, MN, DNA strand breaks or DNA adducts. Smoking caused a significant increase of 1-OHpyr (P<0.05), SCE (P<0.001), HFC (P<0.001) and DNA adducts (P<0.05), but not of MN or DNA strand breaks. Following correction for the smoking-related effects, no occupational induction of the effect biomarkers could be discerned. Multi-variate analysis did not show a significant influence of GSTM1 and GSTT1 polymorphisms on any biomarker. Also no significant interactions were observed between the various biomarkers. Conclusion: This study shows that in the examined plant, the occupational exposure to PAH does not result in measurable early biological effects Copyright © 2001 British Occupational Hygiene Society. Chemicals/CAS: Coke; DNA Adducts; glutathione S-transferase M1, EC 2.5.1.18; glutathione S-transferase T1, EC 2.5.1.-; Glutathione Transferase, EC 2.5.1.18; Polycyclic Hydrocarbons, Aromatic