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Allergic inflammation in the upper respiratory tract of the rat upon repeated inhalation exposure to the contact allergen dinitrochlorobenzene (DNCB).

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Author: Triel, J.J. van · Arts, J.H. · Muijser, H. · Kuper, C.F.
Institution: TNO Kwaliteit van Leven
Source:Toxicology, 1, 269, 73-80
Identifier: 346422
Keywords: Chemistry · 1 chloro 2,4 dinitrobenzene · allergen · autacoid · animal · article · breathing mechanics · comparative study · drug effect · exposure · female · immunology · inhalational drug administration · pathology · pathophysiology · rat · respiratory system · skin allergy · Wistar rat · Administration, Inhalation · Allergens · Animals · Dermatitis, Allergic Contact · Dinitrochlorobenzene · Female · Inflammation Mediators · Inhalation Exposure · Rats · Rats, Wistar · Respiratory Mechanics · Respiratory System


Previously, the contact allergen dinitrochlorobenzene (DNCB) was identified as a sensitizer by inhalation in BALB/c mice; in addition, DNCB induced a lymphocytic infiltrate in the larynx of dermally sensitized Th1-prone Wistar rats upon a single inhalation challenge. In the present study, repeated inhalation exposures to DNCB were investigated using the same protocol as the single-challenge study: female Wistar rats were dermally sensitized with DNCB and subsequently challenged by inhalation exposure to 7 or 15 mg/m(3) DNCB twice a week for 4 weeks. Allergy-related apnoeic breathing was not observed. DNCB-specific IgG antibodies were found in the serum and--predominantly lymphocytic--inflammations were found in the nasal tissues and larynx. Similar effects were observed in animals repeatedly exposed by inhalation without previous dermal contact, indicating sensitization by inhalation. The inflammation may be the upper respiratory tract analogue of hypersensitivity pneumonitis/allergic alveolitis. Possible progression of the airway inflammation upon long-term exposure should be investigated to support or dismiss discrimination between contact and respiratory allergens in relation to respiratory allergy. (c) 2010 Elsevier Ireland Ltd. All rights reserved.